|
|||||||||||||||||||||||||||||||||||||||||||||||||
|
The observation cuvette is one of the most critical parts of all Stopped-Flow instruments. Indeed, it is extremely important to adapt the cuvette to the parameter being observed. For example, it would be inappropriate to use the same cuvette for measuring a small absorbance change and for measuring a fluorescence change of a compound having a high absorbance and producing strong inner filter effects . If our standard cuvettes do not satisfy your specific experimental requirements, we invite you to contact us about custom-made cuvettes.
The observation head has been designed so that the observation cuvettes can be exchanged within a few minutes. This is often recognized by our users as one of the many advantages of the SFM systems.
The table below shows the cuvettes presently available and their specifications. There are two general styles of cuvettes: FC (fluorescence cuvette) FC type cuvettes have blackened edges to reduce light scattering in fluorescence configuration. The best dead time performance are obtained with the FC-08 cuvette while the FC-15 and FC-20 cuvettes are the best choices for CD experiments in the far UV where their large aperture facilitates low noise recording at these wavelengths. TC (transmittance cuvette) TC type cuvettes have been primarily designed for absorbance and transmittance experiments, however in the TC-xx/yyF models, both sides along the light path are transparent. These models of cuvettes can also be used for fluorescence experiments using dilute samples and excitation with a laser or any other low divergence light source. Cuvettes of the TC.xx/10 type have a 1x1 mm² cross section and cuvettes of the TC.xx/15 type have a 1.5x1.5 mm² cross section.
Observation cuvettes - geometry
Notes: |
|||||||||||||||||||||||||||||||||||||||||||||||||
