Rapid-Kinetics and Spectroscopy: Stopped-Flow; Fluorescence, Absorbance, Circular Dichroism, Anisotropy Spectrometry

Recently added articles

Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols
Jeremiah Feducia, Rania Dumarieh, Lauren B. G. Gilvey, Tatyana Smirnova, Stefan Franzen, and Reza A. Ghiladi
Department of Chemistry, North Carolina State UniVersity, Raleigh, North Carolina 27695 Biochemistry 2009, 48, 995–1005
(SFM-400 + freeze quench)

Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH
Hassan Al-Ali, Megan E. Rieger, Kenneth L. Seldeen, Thomas K. Harris, Amjad Farooq and Karoline J. Briegel
Biochemical and Biophysical Research Communications- Volume 391, Issue 1, 1 January 2010, Pages 1104-1109
(SFM-400 + MOS-450AF/CD)

Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols
Jeremiah Feducia, Rania Dumarieh, Lauren B. G. Gilvey, Tatyana Smirnova, Stefan Franzen, and Reza A. Ghiladi Department of Chemistry, North Carolina State UniVersity, Raleigh, North Carolina 27695
Biochemistry 2009, 48, 995–1005 (SFM-400 + DAD)

Kinetics of Complex Formation between DNA and Cationically Charged Cylindrical Brush Polymers Observed by Stopped Flow Light Scattering
Frauke Kuehn, Karl Fischer, Manfred Schmidt
Macromolecular Rapid Communications, 2009, Volume 30 Issue 17, Pages 1470 – 1476
(SFM-300 I Light scattering)

pH (low) insertion peptide (pHLIP) inserts across a lipid bilayer as a helix and exits by a different path
Oleg A. Andreev, Alexander G. Karabadzhak, Dhammika Weerakkody Gregory O. Andreev, Donald M. Engelman , and Yana K. Reshetnyak
PNAS March 2, 2010 vol. 107 no. 9 4081-4086
(SFM-300 + MOS-450AF/CD)

Temperature and Solvent Effects on Radical Scavenging Ability of Phenols
Velmurugan Thavasi, Ryan Phillip Anthony Bettens, and Lai Peng Leong J. Phys. Chem. A, 2009, 113 (13), 3068-3077
(SFM-300 + MOS200 I)

Automated Cell-Based Assay for Screening of Aquaporin Inhibitors
Maria Grazia Mola, Grazia Paola Nicchia, Maria Svelto, David C. Spray and Antonio Frigeri
Anal. Chem., 2009, 81 (19), pp 8219–8229
(SFM-20 + J620 I Light scattering)

Comparative Study of Temperature-Induced Association of Cyclic and Linear Poly(N-isopropylacrylamide) Chains in Dilute Solutions by Laser Light Scattering and Stopped-Flow Temperature Jump
Jing Ye, Jian Xu, Jinming Hu, Xiaofeng Wang, Guangzhao Zhang, Shiyong Liu, Chi Wu
Macromolecules, 2008, 41 (12), pp 4416–4422
(SFM300 + MOS250-mT jump)

A Calibration Reaction for Rapid Freeze-Quench W-Band EPR
A. Potapov; D. Goldfarb
Applied Magnetic Resonance (2010) 37/845-850
(SFM-300+freeze quench)

Binding kinetics of glucose and allosteric activators to human glucokinase reveal multiple conformational states
Antoine M, Boutin J A, Ferry G (2009)
Biochemistry, 2009, 48, 5466-5482
(SFM300 + MOS200M)

Local structure of reaction intermediates probed by time-x-ray absorption near edge structure spectroscopy
G. Smolentsev, G. Guilera, M. Tromp, S. Pascarelli, and A. V. Soldatov
THE JOURNAL OF CHEMICAL PHYSICS 130, 174508 (2009)
(SFM-300 + ED-EXAFS)

The mechanism of addition of pyridoxal 5´-phosphate to Escherichia coli apo-serine hydroxymethyltransferase
Francesca MALERBA, Andrea BELLELLI, Alessandra GIORGI, Francesco BOSSA and Roberto CONTESTABILE
Biochem. J. (2007) 404 (477–485)
(QFM-400)

Distinct Unfolding and Refolding Pathways of Ribonuclease A Revealed by Heating and Cooling Temperature Jumps
Joan Torrent, Stéphane Marchal , Marc Ribó , Maria Vilanova , Cédric Georges , Yves Dupont and Reinhard Lange
Biophysical Journal 94:4056-4065 (2008)
(SFM-300+mT-jump+MOS-200)

Micellization Kinetics of a Novel Multi-Responsive Double Hydrophilic Diblock Copolymer Studied by Stopped-Flow pH and Temperature Jump
Yanfeng Zhang, Tao Wu, Shiyong Liu
Macromol. Chem. Phys. 2007, 208, 2492–2501
(SFM-300+mT-jump+MOS-250)

Key feature of the catalytic cycle of TNF-α converting enzyme involves communication between distal protein sites and the enzyme catalytic core
Ariel Solomon, Barak Akabayov, Anatoly Frenkel, Marcos E. Milla, and Irit Sagi
Proc Natl Acad Sci U S A, 2007 March 20; 104(12): 4931–4936.
(SFM-300+ X-ray)

A semiquinone intermediate generated at the Qo site of the cytochrome bc1 complex: Importance for the Q-cycle and superoxide production
Jonathan L. Cape, Michael K. Bowman, and David M. Kramer
Proc Natl Acad Sci U S A. 2007 May 8; 104(19): 7887–7892
(SFM-3 +EPR)

Biochemical Characterization and Ligand Binding Properties of Neuroglobin, a Novel Member of the Globin Family
Sylvia Dewilde, Laurent Kiger, Thorsten Burmester,Thomas Hankeln, Veronique Baudin-Creuza, Tony Aerts, Michael C. Marden, Roland Caubergs, and Luc Moens
J. Biol. Chem., Vol. 276, Issue 42, 38949-38955, October 19, 2001
(http://www.jbc.org/cgi/content/full/276/42/38949#SEC1) ^note
(SFM-3 + MOS-200)

Characterization of human D-amino acid oxidase
Gianluca Molla, Silvia Sacchi, Mariagrazia Bernasconi, Mirella S. Pilone, Kiyoshi Fukui, Loredano Pollegioni
FEBS Letters 580 (2006) 2358–2364
(http://www.dbsm.uninsubria.it/biochim/publications/papers/2006b_molla.pdf) ^note
(SFM-300+DAD)

Reduction of ferric haemoproteins by tetrahydropterins: a kinetic study
Chantal CAPEILLERE-BLANDIN, Delphine MATHIEU and Daniel MANSUY
Biochem. J. (2005) 392, 583–587
(http://www.biochemj.org/bj/392/0583/3920583.pdf) ^note
(SFM-3 + Diode array)

A Millisecond Infrared Stopped-Flow Apparatus
Jia Tang and Feng Gai
Applied Spectroscopy, Vol. 60, Issue 12, pp. 1477-1481
(SFM-300 + FT-IR accessory)

Covalent heme attachment in Synechocystis hemoglobin is required to prevent ferrous heme dissociation
Julie A. Hoy, Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove
Protein Science (2007), 16:250-260
(SFM-400+MOS-250 | Stopped-Flow & fluorescence)

Multistate reaction kinetics of 6-hydroxy-4-(dimethylamino)flavylium driven by pH. A stopped-flow study
César A. T. Laia, A. Jorge Parola, Filipe Folgosa and Fernando Pina
Org. Biomol. Chem., 2007, 5, 69 - 77
(SFM-300 + DAD | Stopped-Flow diode array)

Specific Aquaporins Facilitate the Diffusion of Hydrogen Peroxide across Membranes
Gerd P. Bienert, Kim A. Kristiansen, Ian M. Møller, and Thomas P. Jahn
J Biol. Chem. 2007 Jan 12;282(2):1183-92
(MOS-250+SFM-300 | Stopped-flow & fluorescence+ light scattering)

Escherichia coli RecQ Is a Rapid, Efficient, and Monomeric Helicase
Xing-Dong Zhang, Ping Xie, Peng-Ye Wang, and Xu Guang Xi
J. Biol. Chem., Vol. 281, Issue 18, 12655-12663, May 5, 2006
(SFM-400 + MOS-450/AF-CD | Stopped-flow+ fluorescence anisotropy)

The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1
Alphée Michelot, Christophe Guérin, Shanjin Huang, Mathieu Ingouff, Stéphane Richard, Natalia Rodiuc, Christopher J. Staiger, and Laurent Blanchoin
Plant Cell. 2005 August; 17(8): 2296–2313
(MOS-450/AF-CD | slow kinetics fluorescence)

RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer
Xing-Dong ZHANG, Shuo-Xing DOU, Ping XIE, Peng-Ye WANG, and Xu Guang
XI1 Acta Biochim Biophys Sin 2005,37:593-600
(SFM-400+MOS-450/AF-CD | Stopped-flow + FRET fluorescence)

Revisiting a proposed kinetic model for the reaction of cysteine and hydrogen peroxide via cysteine sulfenic acid
Michael T. Ashby , Péter Nagy
International Journal of Chemical Kinetics; Volume 39, Issue 1 , Pages 32 - 38
(SFM-400+MOS-450/AF | Stopped-flow + fluorescence/absorbance)

Structural Characterization of Apomyoglobin Self-Associated Species in Aqueous Buffer and Urea Solution
Charles Chow , Nese Kurt *, Regina M. Murphy and Silvia Cavagnero
Biophysical Journal 90:298-309 (2006)
(MOS-450/AF-CD + SFM-400 | CD stopped-flow and CD steady state mode)

In situ investigation of the oxidative addition in homogeneous Pd catalysts by synchronised time resolved UV-Vis/EXAFS
Gemma Guilera*^a, Mark A. Newtona, Charlene Pollia, Sakura Pascarellia, Meritxell Guinób and King Kuog (Mimi) Hii*^b
a) European Synchrotron Radiation Facility (ESRF, ID24), P.O. Box 220, 38043 Grenoble Cedex, France. E-mail: guilera@esrf.fr; Fax: +33 (0)4 7688 2784; Tel: +33 (0)4 3888 1930
b) Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, UK SW7 2AZ. E-mail: mimi.hii@imperial.ac.uk; Fax: +44 (0)20 7594 5804; Tel: +44 (0)20 7594 1142
Chem. Commun., 2006, DOI: 10.1039/b606772g
(SFM-300+Diode array+EXAFS)

Amyloid formation under physiological conditions proceeds via a native-like folding intermediate
Thomas R Jahn, Martin J Parker, Steve W Homans & Sheena E Radford
Nature, Structural and Molecular Biology, 13, 195 - 201 (2006)
(SFM-4 + MOS-450/AF-CD)

Polymerization of Anionic Wormlike Micelles
Zhiyuan Zhu, Yamaira I. Gonzalez, Hangxun Xu, Eric W. Kaler, and Shiyong Liu
Langmuir 2006, 22, 949-955
(SFM-300/S + MOS-250, light scattering)

pH-Induced Micellization Kinetics of ABC Triblock Copolymers Measured by Stopped-Flow Light Scattering
Zhiyuan Zhu, Steven P. Armes, and Shiyong Liu
Macromolecules 2005, 38, 9803-9812
(SFM-300/S + MOS-250, light scattering)

A Stopped-Flow Kinetic Study of the Assembly of Interpolymer Complexes via Hydrogen-Bonding Interactions
Shizhong Luo, Shiyong Liu, Jian Xu, Hao Liu, Zhiyuan Zhu, Ming Jiang, and Chi Wu
Macromolecules, 2006; 39(13); 4517-4525
(SFM-300/S + MOS-250, light scattering)

First Observation of Two-Stage Collapsing Kinetics of a Single Synthetic Polymer Chain
Jian Xu, Zhiyuan Zhu, Shizhong Luo, Chi Wu, and Shiyong Liu
Physical Review Letters, 2006, 96, 027802
(SFM-300/S + MOS-250, light scattering, fluorescence)

Mechanism of a-oxoamine synthases: identification of the intermediate Claisen product in the 8-amino-7-oxononanoate synthase reaction
Olivier Kerbarh, Dominic J. Campopiano and Robert L. Baxter*
Chem. Commun., 2006, 60–62
(MOS-450/AF-CD + SFM-300)

Probing the Kinetics of Membrane-Mediated Helix Folding
Matthew J. Tucker, Jia Tang, and Feng Gai
J. Phys. Chem. B, 110 (15), 8105 -8109, 2006
(SFM-300+ microcuvette (fluorescence + FRET))

Efficient Refolding of Aggregation-prone Citrate Synthase by Polyol Osmolytes HOW WELL ARE PROTEIN FOLDING AND STABILITY ASPECTS COUPLED?*
Rajesh Mishra, Robert Seckler, and Rajiv Bhat
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 280, No. 16, Issue of April 22, pp. 15553-15560
(SFM-300 + J-810)

Time-resolved scattering investigations of brome mosaic virus microcrystals appearance
M. Casselyn, S. Finet, A. Tardieu and H. Delacroix
Acta Cryst. (2002). D58, 1568-1570
(SFM-3 + small angle X-ray scattering)

Ribose 2'-hydroxyl groups in the 5' strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation
JASON S. FEINBERG and SIMPSON JOSEPH
RNA (2006), 12:580-588
(SFM-20 + MOS-200)

The mechanism of GroEL/GroES folding/refolding of protein substrates revisited
Huw Jones, Monika Preuss, Michael Wright and Andrew D. Miller
Organic & Biomolecular Chemistry, 2006, 4, 1223 - 1235
(SFM-3 + MOS-200)

Trifluoroethanol-Induced Unfolding of Concanavalin A: Equilibrium and Time-Resolved Optical Spectroscopic Studies
Qi Xu and Timothy A. Keiderling
Biochemistry, 44 (22), 7976 -7987, 2005
(SFM-400 + J-810)

Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis
Laura Caldinelli, Gianluca Molla, Mirella S. Pilone and Loredano Pollegioni
FEBS journal - Volume 273 Page 504 - February 2006
(SFM-300 + Jasco FP-750)

Structural and kinetics characterization of the simplified SH3 domain FP1
Q.Yi, P.Rajagopal, R.Klevit, D.Baker
Protein Science (2003), 12: 776-783
(SFM-4 + MOS-200)

Stabilization of Native Protein fold by intein-mediated covalent cyclization
N.Williams, E.liepinsh, S.Watt, P.Prosselkov, J.Matthews, P.Attard, J.Beck, N.Dixon, G.Otting
L.Mol.Biol.(2005) 346, 1095-1108
(SFM-300+J-810)

Computational redesign of protein-protein interaction specificity
T.Kortemme, L.Joachimiak, A.Bullock, A.Schuler, B.Stoddard, D.Baker
Nature Structural and Molecular Biology, Volume 11, Number 4, April 2004, 372-379
(SFM-4 + SPEX fluorolog)

Evidence of Two Distinct Oxygen Complexes of Reduced Endothelial Nitric Oxide Synthase
Stéphane Marchal, Morten Sørlie, K. Kristoffer Andersson, Bernd Mayer, and Reinhard Lange
J. Biol. Chem., Vol. 279, Issue 19, 19824-19831, May 7, 2004
(SFM-300+DAD+MOS-200)

Pore selectivity analysis of an aquaglyceroporin by stopped-flow spectrophotometry on bacterial cell suspensions.
Hubert JF, Duchesne L, Delamarche C, Vaysse A, Gueune H, Raguenes-Nicol
C. Biol Cell. 2005 Sep;97(9):675-86.
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15859950&query_hl=1&itool=pubmed_docsum)  ^note  
(SFM-300+MOS-250)

Probing the Reorganization of the Nicotinic Acetylcholine Receptor during Desensitization by Time-Resolved Covalent Labeling Using [3H]AC5, a Photoactivatable Agonist
 Alexandre Mourot,1 Jordi Rodrigo,2 Florence Kotzyba-Hibert, Sonia Bertrand, Daniel Bertrand, and Maurice Goeldner
 Mol Pharmacol 69:452–461, 2006.
(http://molpharm.aspetjournals.org/cgi/reprint/69/2/452)  ^note
(SFM-300 + optical quench option)

RecQ Helicase-catalyzed DNA unwinding detected by fluorescence resonance energy transfer
X-D.Zhang, S-X.Dou, P. Xie, P-Y Wang, X-G Xi
Acta biochimica et Piophysica Sinica 2005, 37(9): 593-600.
(http://www.abbs.info/pdf/37-09/3-593-600-05074.pdf)  ^note
(MOS-450 AF/CD + SFM-400 fluorescence)

The Heparin Binding Properties of Heparin Cofactor II Suggest an Antithrombin-like Activation Mechanism
Denis O'Keeffe, Nijole Gasiunas, John Gallagher, Trevor P. Baglin, and James A. Huntington J.
Biol. Chem., Vol. 279, Issue 48, 50267-50273, November 26, 2004
(MOS-450+SFM-300 : fluorescence)

Rapid mixing methods for exploring the kinetics of protein folding
H.Roder, K.maki, H.Cheng, M Ramachandra Shastry
Methods 34 (2004) 15-27.
(http://www.fccc.edu/research/labs/roder/pdf/RoderMethods04.pdf)
(SFM-400+ microcuvette: dead time of 0.55 ms)

Cooperative Sub-Millisecond Folding Kinetics of Apomyoglobin pH 4 Intermediate
Sebastien Weisbuch, Francine Gérard, Marielle Pasdeloup, Jéremy Cappadoro, Yves Dupont, and Marc Jamin
Biochemistry 2005, 44, 7013-7023.
(http://pubs.acs.org/cgi-bin/article.cgi/bichaw/2005/44/i18/pdf/bi047372v.pdf)  ^note
(MOS-450AF/CD + SFM-400+microcuvette)

Dynamics of structural transitions in amphiphilic systems monitored by scattering techniques
M. Gradzielski, I. Grillo, T. Narayanan
Prog Colloid Polym. Sci. (2004) 129: 32-39

An advanced EPR stopped-flow apparatus based on a dielectric ring resonator
Günter Lassmann, Peter Paul Schmidt and Wolfgang Lubitz
Journal of Magnetic Resonance, Volume 172 Issue 2 , February 2005, Pages 312-323

Stopped-flow and Mutational Analysis of Base Flipping by the Escherichia coli Dam DNA-(adenine- N6)-methyltransferase.
Kirsten Liebert, Andrea Hermann, Martina Schlickenrieder and Albert Jeltsch.
J. Mol. Biol. (2004) 341, 443-454
(SFM-3 + MOS-200)

Steps towards Phage Display Libraries with an extended amino acid repertoire
M. Björklund, E. Koivonen
Letters in Drud Design and Discovery, 2004, 1, 163-167
(MOS-250 emission fluorescence)

DNA recognition by the Homing Endonuclease PI-SceI involves a divalent metal ion cofactor induced conformational change
AJ Noel, W. Wende, A.Pingoud
Journal of Biological Chemistry, Vol 279, N°8, 6794-6804, 2004
(SFM-300 + fluorescence)

Apo-azurin folds via an intermediate resembles the moten-globule
A.Sandberg, J.Leckner, BG. Karlsson
Protein Science (2004), 13, 2628-2638
(SFM-20 + MOS-250 + MOS-450 in fluorescence and CD mode)

Probing the influence on folding behavior of structurally conserved core residues in P.Aerogonesa apo-azurin
K.C.Engman, A. Sandberg, J.Leckner, G. Karlsson
Protein Science (2004), 13, 2706-2715
(SFM-20 + MOS-450 in fluorescence mode)

Acid-base behavior of organopalladium complexes [Pd(CNN)R]BF4
M.S.Munoz, B.Garcia, S.Ibeas, F.J.Hoyuelos,I.Penacoba, A.M.Navarro,J.M.Leal
New.J.Chem., 2004, 28, 1450-1456
(SFM-300 + diode array)

Two coupled photochromic systems of 3',4'-(methylendioxy)flavilium : kinetic and thermodynamic characterisation
D.Fernàndez, F.Folgosa, A.J.Parola, F.Pina
New.J.Chem., 2004, 28, 1221-11226
(SFM-300 + diode array)

Properties of some variants of human beta2-microglobulin and amyloidogenesis
A.Corazza,F.Pettirossi, P.Viglino, G.Verdone, J.Garcia, P.Dumy, S.Giorgetti, P.Mangione, S.raimondi, M.Stoppini, V.belloti, G.Esposito
Journal of Biological Chemistry, Vol.279, N°10, 9176-9189, 2004
(SFM-300 + fluorescence )

Roles of conserved P domain residues and Mg2+ in ATP binding in the ground and Ca2+ activated states of sarcoplasmic reticulum Ca2+ - ATPase
D.B.McIntosh, J.D.Clausen, D.G. Woolley, D.H.MacLennan, B.Vilsen, J.P.Andersen
Journal of biological Chemistry, vol.279, (2004)
QFM-5 (double mixing)

Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum Ca2+ - ATPase assists in catalysis of E2/E2P partial reactions
J.D.Clausen, B.Vilsen, D.B.McIntosh, A.P.Einholm, J.P. Andersen
PNAS, 2776-2781, march 2004, vol.101, n° 9
QFM-5 and SFM-400/Q (double mixing)

Importance of transmembrane segment M1 of the sarcoplasmic reticulum Ca2+-ATPase in Ca2+ occlusion and phosphoenzyme processing
A.P.Einholm, B.Vilsen, J.P.Andersen
Journal of biological Chemistry, vol.279, n°16, (2004), 15888-15896
QFM-5 and SFM-400/Q (double mixing)

Secondary structure formation is the earliest structural event in the refolding of an all beta-sheet protein
T.Sivaraman, T.Kumar, Y.Tu, W.Wang, W.Lin, H.Chen, C.Yu
Biochemical and Biophysical research communications, 260, 284-288 (1999).
(http://www.uark.edu/depts/cheminfo/uarkchem/facultystaff/faculty/Yu/A70.pdf)  ^note
(SFM-3+J-720)

Roles of Leu249, Lys252 and Leu253 in membrane segment M3 of sarcoplasmic reticulum Ca2+-ATPase in control of Ca2+ migration and Long-range intramolecular communication
J.D.Clausen, J.P.Andersen
Biochemistry (2003), 42, 2585-2594
QFM-5 and SFM-400/Q (double mixing)

Dissection of the functionnal differences between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 2 isoforms and characterization of darier disease (SERCA2)mutants by steady-state and transient kinetic analysis
L.Dode, J.P.Andersen, N. Leslie, J.Dhitavat, B.Vilsen, A.Hovnanian
Journal of biological Chemistry, vol.278, n°48, (2003), 47877-47889
QFM-5 (double mixing)

Importance of conserved N-domain residues thr441, Glu442, lys515, Arg560, Leu562 of sarcoplasmic reticulum Ca2+-ATPase for MgATP binding and subsequent catalytic steps
J.D.Clausen, D.B. McIntosh, B.Vilsen, D.G.Woolley, J.P. Andersen
Journal of biological Chemistry, vol.278, n°22, (2003), 20245-20258
QFM-5 (double mixing)

Importance of conserved Thr214 in domain A of the Na+, K+ - ATPase for stabilization of the phosphoryl state complex in E2P dephosphorylation
M. Toustrup-Jensen, B. Vilsen
Journal of biological Chemistry, vol.278, n°19, (2003), 11402-11410
QFM-5 (double mixing)

Functional consequences of alterations of Ile279, Ile283, Glu284, his285, Phe286, and His288 in the NH2-terminal part of the transmembrane helix M3 of the Na+, K+ - ATPase
M. Toustrup-Jensen, B. Vilsen
Journal of biological Chemistry, vol.278, n°40, (2003), 38653-38664
QFM-5 (double mixing)

Dissection of the functionnal differences between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 3 isoforms by steady-state and transient kinetic analysis
L.Dode, B.Vilsen, K. Van Baelen, F. Wuytack, J.D. Clausen, J.P.Andersen
Journal of biological Chemistry, vol.277, n°47, (2002), 45579-45591
QFM-5 (double mixing)

Importance of Glu282 in transmembrane segment M3 of the of the Na+,K+-ATPase for control of the cation interaction and conformational changes
M. Toustrup-Jensen, B. Vilsen
Journal of biological Chemistry, vol.277, n°41, (2002), 38607-38617
QFM-5 (double mixing)

Importance of transmembrane segment M3 of the sarcoplasmic reticulum Ca2+-ATPase for control of the gateway to the Ca2+ sites
J.P. Andersen, T.Lykke-Moller Sorensen, K. Povlsen, b. Vilsen
Journal of biological Chemistry, vol.276, n°26, (2001), 23312-23321
QFM-5 (double mixing)

Importance of Thr-353 of the conserved phosphorylation loop of the sarcoplasmic reticulum Ca2+-ATPase in MgATP binding and catalytic activity
J.D. Clausen, D.B. McIntosh, D.G.Woolley, J.P.Andersen
Journal of biological Chemistry, vol.276, n°38, (2001), 35741-35750
QFM-5 (double mixing)

Mutational effects on conformational changes of the dephospho-and phospho-forms of the Na+,K+-ATPase
M. Toustrup-Jensen, M. Hauge, B. Vilsen
Biochemistry (2001), 40, 5521-5532
QFM-5 (double mixing)

Importance of stalk segment S5 for Intramolecular communication in the sarcoplasmic Ca2+-ATPase
Thomas Lykke-Moller Sorensen, Jens Peter Andersen
Journal of biological Chemistry, vol.275, n°37, (2000), 28594-28961
QFM-5 (double mixing)

'Facile Variation of Reagent Concentrations in Rapid Quench Enzymology.
Sohn, J. and Rudolph, J. (2003)  Anal. Biochem. 312, 80-83
(QFM-400 microvolume Quench-Flow)

The signal flow and motor response controling chemotaxis of sea urchin sperm.
Kaupp U.B., Solzin J., Hildebrand E., Brown, J.E., Helbig A., Hagen V., Beyermann M., Pampaloni F., Weyand I. 
Nature Cell Biology 5 (2003) S. 109-117
(SFM-4/S + MOS-200 | Fluorescence)

Structural reorganization of the Acetylcholine binding site of the Torpedo nicotinic receptor as revealed by dynamic photoaffinity labeling
Thomas Grutter, Sonia Bertrand, Florence Kotzyba-Hibert, Daniel Bertrand, Maurice Goeldner
ChemBioChem 2002, 3, 652-658
(SFM-300 + special optical quench option)

Enzymatic mechanism of RNA translocation in dsRNA bacteriophages.
Lisal, J., Kainov, D. E., Bamford, D. H., Thomas, G. J., Jr., and Tuma R.
(2004). J. Biol. Chem., 279, 1343-1350
(SFM-20 + MOS-250 | Fluorescence )

Amino Acids of the Bacterial Toxin SopE Involved in G Nucleotide Exchange on Cdc42
Markus C. Schlumberger <l >, Gretel Buchwald, Klaus Scheffzek , Alfred Wittinghofer , and Wolf-Dietrich Hardt
J. Biol. Chem., Vol. 278, Issue 29, 27149-27159, July 18, 2003
(SFM-20 + MOS-450 AF/CD | SF Fluorescence and steady state CD spectra)

The influence of hinge region residue Glu381 on antithrombin allostery and metastability.
Johnson DJ, Huntington JA.
J Biol Chem. 2003 Nov 17.
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14623882&dopt=Abstract)  ^note
(SFM-300 & MOS-450 in fluorescence)

Catalytic Mechanism of the Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26.
Prokop, Z., Monincova, M., Chaloupkova, R., Klvana, M., Nagata, Y., Janssen, D.B., Damborsky, J.
2003: Journal of Biological Chemistry 278: 45094-45100.
(QFM-400 microvolume Quench-Flow & SFM-20+J-810 in Fluo)

Kinetic Analysis of R67 Dihydrofolate Reductase Folding: From the Unfolded Monomer to the Native Tetramer
Christophe Bodenreider, Nicolas Kellershohn, Michel E. Goldberg, and Annick Méjean
Biochemistry ; 2002; 41(50); 14988-14999.
(http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2002/41/i50/abs/bi020453b.html)  ^note
(SFM-300 + MOS components + Jobin-Yvon CD6)

Kinetic Characterization of the pH-Dependent Oligomerization of R67 Dihydrofolate Reductase
Mejean, A.; Bodenreider, C.; Schuerer, K.; Goldberg, M. E.
Biochemistry ; 2001; 40(27); 8169-8179.
(http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2001/40/i27/abs/bi010611j.html) ^note
(SFM-300 + MOS components + Jobin-Yvon CD6)

Role of individual disulfide bonds in hen lysozyme early folding steps Valérie Guez, Pascale Roux, Amiel Navon and Michel E. Goldberg
Protein Science (2002), 11:1136-1151.
(http://www.proteinscience.org/cgi/content/abstract/11/5/1136) ^note
(SFM-300 + MOS components + Jobin-Yvon CD6)

Immunochemical pulsed-labeling characterization of intermediates during hen lysozyme oxidative folding Nicole M. Jarrett1, Lisa Djavadi-Ohaniance, Richard C. Willson, Hideki Tachibana and Michel E. Goldberg Protein
Science (2002), 11:2584-2595.
(http://www.proteinscience.org/cgi/content/abstract/11/11/2584) ^note
(SFM-300 + MOS components + Jobin-Yvon CD6)

Formation and growth of anionic vesicles followed by small angle neutron scattering
I. Grillo, E.I. Kats, A.R. Muratov
Langmuir (2003), 19 (11) 4573-4581
(SFM-3 & ILL neutron beam)

The complex between a four-way DNA junction and T7 endonuclease 1
A.C.Déclaix, J.M.Fogg, A.D.J.Freeman, F. Coste, J.M.Hadden, S.E.V.Phillips and David M.J.Lilley
EMBO vol 22 no.6 pp 1398-1409 2003
(QFM-400 microvolume Quench-Flow)

Mapping the Cytochrome c Folding Landscape
Julia G. Lyubovitsky, Harry B. Gray,* and Jay R. Winkler*
J. AM. CHEM. SOC. 2002, 124, 5481-5485
(http://pubs.acs.org/cgi-bin/abstract.cgi/jacsat/2002/124/i19/abs/ja017399r.html)  ^note
(SFM-4 : double kinetics fluorescence lifetime)

Surface Expansion Is Independent of and Occurs Faster than Core Solvation during the Unfolding of Barstar
K. Sridevi and Jayant B. Udgaonkar*
Biochemistry 2003, 42, 1551-1563
(http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2003/42/i06/abs/bi0268697.html)  ^note
(SFM-400 et MOS-250 in fluorescence)

DNA: REPLICATION REPAIR AND RECOMBINATION: Kinetic Mechanism for the Formation of the Presynaptic Complex of the Bacterial Recombinase RecA
Martine Defais, Emilie Phez, and Neil P. Johnson
J. Biol. Chem., Jan 2003; 278: 3545 - 3551
(http://www.jbc.org/cgi/content/abstract/278/6/3545)  ^note
(MOS-400, Steady state or slow kinetics recordings)

Characterization of Single-Tryptophan Mutants of Histidine-Containing Phosphocarrier Protein: Evidence for Local Rearrangements during Folding from High Concentrations of Denaturant
Azuaga, A. I.; Canet, D.; Smeenk, G.; Berends, R.; Titgemeijer, F.; Duurkens, R.; Mateo, P. L.; Scheek, R. M.; Robillard, G. T.; Dobson, C. M.; van Nuland, N. A. J.;
Biochemistry ; (Article); 2003; 42(17); 4883-4895.
(http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2003/42/i17/abs/bi027182p.html) ^note
(Stopped-Flow/CD with SFM-3 & MOS-400, Stopped-Flow/Fluorescence&Fluorescence Anisotropy with SFM-3 &  MOS-400 using the EMFA^® method)

Phosphorylation of the WASP-VCA Domain Increases Its Affinity for the Arp2/3 Complex and Enhances Actin Polymerization by WASP
Giles O.C. Cory, Rainer Cramer, Laurent Blanchoin, and Anne J. Ridley
Molecular Cell, Vol. 11, 1229–1239, May, 2003
(steady state fluorescence with MOS-450)

Metal ions bound at the active site of the junction-resolving enzyme T7 endonuclease I.
Hadden JM, Declais AC, Phillips SE, Lilley DM.
EMBO J 2002 Jul 1;21(13):3505-15
(QFM-400 microvolume Quench-Flow)

Understanding protein hydrogen bond formation with kinetic H/D amide isotope effects
Bryan A. Krantz1, Alok K. Srivastava, Sehat Nauli, David Baker, Robert T. Sauer & Tobin R. Sosnick
Nature Structural Biology June 2002 Volume 9, pp 458 - 463
(SFM-4/S et Q)

Local cooperativity in the unfolding of an amyloidogenic variant of human lysozyme
Denis Canet, Alexander M. Last, Paula Tito, Margaret Sunde, Andrew Spencer, David B. Archer, Christina Redfield, Carol V. Robinson & Christopher M. Dobson
Nature structural Biology (2002) vol 9, pp 308 - 315
(QFM-5 coupled to mass spectrometry)

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