Electrophysiology: Micro Electrode Array (MEA) system, solution changer, puller, micromanipulator |
|
|
||||||||||||||||||||||
|
RSC-200 (and RSC-160) Software upgrade Click here to download the current version. Then create an empty directory, unzip the downloaded file in this directory and run [setup.exe]. Version history
Electrovalve array (EVH-9) starting instructions Setting up First of all, the reservoir syringes, inserted in the syringe rack has to be fixed on any type of the support which permits a relatively easy adjustment of the height. The height of the rack determines a flow rate of the solutions. In general a syringe rack holder (a vertical metal bar, diameter of 15mm) is used. On the same rack the EVH-9 module can be fixed, below the syringe rack. Tubing The EVH-9 electrovalve array can work with tubing of different types, like silicone, or C-Flex. The outside diameter should not exceed 2.5mm. Also the wall rigidity has to be taken into account, a tubing to hard to be squeezed by the valve spring, may result the valve leakage. Our preference is C-Flex tubing because of its superior impermeability to gasses (ID: 0.5mm, OD: 2.1mm ; COLE PARMER ref : 6424-59 ), normally delivered with the system.
Make sure that the tubing is not stretched between the syringe and the valve, what may block the flow or cause the leakage. Flow test Having installed all tubing in the valves, fill the syringes with water and check all valves one by one. Open the valve by control unit or by hand and see if the water is flowing correctly, and stops when the valve is closed. In case of any problem check : - the tubing is not blocked on the level of luer lock, - the tubing is not under tension between the syringe and the valve, - the tubing is well in the valve groove - the tubing is not blocked inside (use a syringe piston to push the solution) Rapid Solution Changer improvements New head for RSC-100, RSC-160 and RSC-200 We have developed a new head - the "straight head" - which is the head by default for the RSC-160 and RSC-200 supplied from January 1998. The straight head was designed to carry standard capillary glass, OD 1.00mm.
This head has significant advantages over the others:
You can upgrade easily your RSC-100, RSC-160 or RSC-200 with this new head. Note: Glass with outside diameter different from 1.00 mm can be used with the RSC-160 or RSC-200 and the V1.02 version firmware (or higher). For more information about this head, click here.
Better shielding and new motor for RSC-160 & RSC-200 Since January 1998, the shielding of the stepping motor is connected to the RSC-160 & RSC-200 through a pin of the connector rather than using the connector itself. We have indeed found some connectors with loose connection. If you have encountered such a problem, we recommend that you use a wire to set a good contact between the stepping motor and the RSC-160 & RSC-200. Since January 1998, the RSC-160 & the RSC-200 are supplied with a new and compatible stepping motor. This motor has important advantages over the previous version:
As the new stepping motor is compatible with the previous version, you can upgrade your system easily. A shielding wire is supplied for connection to RSC-160 & RSC-200 delivered before January 1998.
New firmware for RSC-160 & RSC-200 To get the version number of your RSC-160 & RSC-200 firmware, keep the "STATUS" button pressed on the front panel. The firmware upgrade consists of the exchange of 2 x EPROM chips of the latest version (operation not warrantied if not done in factory).
Version history
New manual for the MSC-200 (May 2003) Click here for download Preparing the straight head for an experiment Mounting the capillaries 1) Check the capillaries with a calliper for their OD variation Alignment of capillaries Push capillaries out...
...and then use a straight and clean surface to align them.
Check their parallel position and put the scotch or parafilm around the head and capillaries (between joints) to immobilise glass tubes in their position. Mounting the head on the motor Insert entirely the motor axis. Tighten the black screw clockwise (with the tip in front of you), using a fork wrench. To keep the head axis on place when screwing, the hole for grounding can be useful. Head grounding Use the thin cable and the provided pin to connect the head to the closest grounding. High Flow-Rate set-up If a high flow rate is required, you can connect directly the glass capillaries to the large flexible tubing (the polyethylene tubing is then not used). In this case, however, the rear glass end must be bent to give necessary space for tubing: place the capillary just above a flame and wait until the capillary bends downwards under its own weight (30° angle and 1cm length approximately).
|
TOP ![]() |
| Contact us - Legal notice - Chinese reg.num. (京ICP备06015605号) - Last modified: October 20, 2009 - © 2004-2009 Bio-Logic SAS |